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Positioning checks out had been blocked to have best sets, arranged, and you may PCR duplicates removed having fun with Samtools68. Biological replicates had been normalised to RPKM inside a strand-particular trend using deepTools69 bamCoverage as well as the banner –filterRNAstrand. Physiological replicates were merged and changed into bigWig data format for visualisation on the UCSC genome internet browser. Poly(A)- trials was purified using Agencourt RNAClean XP beans (Beckman Coulter) and you will eluted in the RNase totally free liquid.
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